Unusual nucleotide sequences at the 5' end of actin genes in Dictyostelium discoideum.

Characterization of actin variants in Dictyostelium discoideum

Isolation of an Actin-binding Dictyostelium discoideum

We prepared a probe of radiolabeled, glutaraldehyde cross-linked filamentous actin (F-actin) to study binding of actin to membranes of Dictyostelium discoideum. The probe bound to membranes or detergent extracts of membranes with a high affinity and in a saturable manner. The binding could be reduced by boiling of either the actin probe or the membranes, or by addition of excess native F-actin,...

Novel actin rods appeared in spores of Dictyostelium discoideum.

When spores of Dictyostelium discoideum matured through normal development were stained with rhodamine-phalloidin, rod-shaped fluorescence was found across the nucleus and in the cytoplasm. Electron micrographs of the rod shape showed that it consisted of long and straight fibers 12 nm in diameter. In contrast, a conventional actin rod, which usually is formed under stress conditions, is compos...

A Continuum Model of Actin Waves in Dictyostelium discoideum

Actin waves are complex dynamical patterns of the dendritic network of filamentous actin in eukaryotes. We developed a model of actin waves in PTEN-deficient Dictyostelium discoideum by deriving an approximation of the dynamics of discrete actin filaments and combining it with a signaling pathway that controls filament branching. This signaling pathway, together with the actin network, contains...

NH2-terminal processing of Dictyostelium discoideum actin in vitro.

When Dictyostelium discoideum actin is synthesized in a rabbit reticulocyte lysate system, it is made as a 43,000-dalton polypeptide with the NHz-terminal sequence Ac-Met-Asp-Gly . . . even though in vivo the NHz-terminal sequence is Ac-Asp-Gly . . . (Rubenstein, P., Smith, P., Deuchler, J., and Redman, K. (1981) J. Biol. Chem 256,8149-8155). Here we describe the subsequent fate of the NHz-term...